Summary
Type: SPE
Application #: 301050
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
1 column volume methanol, then 1 column volume methanol / water (5 : 95, v/v),
finally 1 ml buffer pH 5 (0.1 M potassium dihydrogen phosphate / 1.34 M
potassium chloride)
Sample application:
Slowly force or aspirate 1 ml urine through the column
Column washing:
3 x 2 ml methanol / water (5 : 95, v/v); dry column under vacuum
Elution:
2 ml methanol
Authors: Macherey-Nagel
Source: LC Department